Protein Method Blocking Buffers
LICOR Intercept (TBS) Blocking Buffer and Diluent Kit
Try Intercept (TBS) Blocking Buffer and Intercept T20 (TBS) Antibody Diluent for improved results in your quantitative Western blots and other immunoassays. Intercept Blocking Buffers provide excellent blocking performance with low background and low variability. Intercept T20 Antibody Diluents improve the specificity of the primary and secondary antibodies, reducing off-target effects. They are preformulated with Tween 20. There’s no need to mix the diluent yourself, which saves you time and reduces potential variation. This kit includes Intercept (TBS) Blocking Buffer (1 x 500 mL) and Intercept T20 (TBS) Antibody Diluent (2 x 500 mL)
LICOR Intercept (PBS) Protein-Free Blocking Buffer (3x500mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Diagnostic Biosystems BACKGROUND BLOCKER Background Blocker (1X)
To block nonspecific background staining observed during immunostaining.\n\nBackground Blocker is unique serum-free blocking solution used to reduce nonspecific background staining. It is a universal blocking agent which eliminates the need to match species with the link antibody. This blocking reagent has been shown to be more superior than serum-based blocking solutions. In addition to immunihistochemistry, Background Blocker can also be used for ELISA and Western Blot techniques.
Abcam Blocking Buffer 10X, 50ML
Blocking Buffer 10X (ab210904) is part of the reagents, controls & accessories range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences OmniPur(R) Nonfat Powdered Milk | 10GM
OmniPur(R) Nonfat Powdered Milk | 10GM
Teknova 1X PBS with 0.05% Tween-20, pH 7.4. 20L Carboy, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
MilliporeSigma™ Novagen™ 5% Alkali - Soluble Casein
Used as blocking reagent for Western blot applications.
BBS, MP Biomedicals™
Serves as a general purpose loading dye. MP Biomedicals BBS can be used with neutral DNA or RNA agarose gels.
MilliporeSigma™ Immunoblot Blocking Reagent
For use in western blots as a blocking agent
Bioworld TBS/Casein, 100 mL
All blocks and dlluents are supplied in a 10X concentration of physiological saline buffer. pH range: 7.6 +/- 0.1
LI-COR Intercept (PBS) Blocking Buffer (25 x 500 mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. Contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies; Excellent choice for biotin-streptavidin detection; Suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays.Formulation: Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes. Shake well before each use.